Demystified ... DNA nucleotide sequencing
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چکیده
منابع مشابه
Demystified ... DNA nucleotide sequencing.
The determination of the nucleotide sequence of a gene is often the ultimate goal of a research project and the frequency with which new sequences are described in the literature and computer databases is remarkable. The basic techniques that underpin these endeavours are remarkably simple and their description now frequently forms part of the very earliest portion of a biological science under...
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Using a first-principles linear response theory, the capacitance of the DNA nucleotides, adenine, cytosine, guanine, and thymine, are calculated. The difference in the capacitance between the nucleotides is studied with respect to conformational distortion. The result suggests that although an alternate current capacitance measurement of a single-stranded DNA chain threaded through a nanogap el...
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DNA sequencing by synthesis during a polymerase reaction using laser-induced fluorescence detection is an approach that has a great potential to increase the throughput and data quality of DNA sequencing. We report the design and synthesis of a photocleavable fluorescent nucleoside triphosphate, one of the essential molecules required for the sequencing-by-synthesis approach. We synthesized thi...
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Osmium tetroxide, 2,2'-bipyridine (Os,bipy) has been widely applied as a probe of the DNA structure. To obtain information about reactivity of DNA bases toward this probe synthetic homopolynucleotides poly(dT), poly(dC), poly(dG) and poly(dA) were treated with Os,bipy and the content of modified bases measured by stripping voltammetry and absorption spectrophotometry. After 20 hours' treatment ...
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ژورنال
عنوان ژورنال: Molecular Pathology
سال: 1999
ISSN: 1366-8714
DOI: 10.1136/mp.52.3.117